BUB1B Rekombinanter Antikörper

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• KD/KO Validated

BUB1B Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240969F7

Kat-Nr. : 83920-6-PBS

Synonyme

BubR1, 240969F7, Bub1A, BUB1beta, EC:2.7.11.1

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  WB analysis using 83920-6-RR (same clone as 83920-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83920-6-RR (BUB1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83920-6-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 83920-6-RR (same clone as 83920-6-PBS)

  WB result of BUB1B antibody (83920-6-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BUB1B transfected HeLa cells. This data was developed using the same antibody clone with 83920-6-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 83920-6-RR (same clone as 83920-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 83920-6-RR (BUB1B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83920-6-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 83920-6-RR (same clone as 83920-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BUB1B antibody (83920-6-RR, Clone: 240969F7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 83920-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83920-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83920-6-RR against Human BUB1B were performed. The affinity constant is 7.60 nM.

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Geprüfte Anwendungen

Produktinformation

83920-6-PBS bindet in WB, IHC, IF/ICC, ELISA BUB1B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: BUB1B fusion protein Ag34182
• Vollständiger Name: budding uninhibited by benzimidazoles 1 homolog beta (yeast)
• Berechnetes Molekulargewicht: 1050 aa, 120 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC018739
• Gene symbol: BubR1
• Gene ID (NCBI): 701
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BUB1B(Mitotic checkpoint serine/threonine-protein kinase BUB1 beta), also named as BUBR1, MAD3, and SSK1, belongs to the BUB1 subfamily. Human BUB1B is a 120 kDa protein that plays a central role in the spindle assembly checkpoint (SAC), the evolutionary conserved and self-regulatory system of higher organisms that monitors and repairs defects in chromosome segregation in mitotic cells(PMID: 22201036). In addition to phosphorylation, BUB1B is also subjected to posttranslational modifications, including acetylation(PMID: 22167194). Defects in BUB1B are the cause of the premature chromatid separation trait (PCS) and mosaic variegated aneuploidy syndrome type 1 (MVA1). Research has shown that a BUB1B isoform with a measured MW of about 65 kDa (PMID: 34620840).