BUB3 Rekombinanter Antikörper

BUB3 Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240087D9

Kat-Nr. : 83266-5-PBS

Synonyme

hBUB3, BUB3L, BUB 3, 240087D9

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  WB analysis using 83266-5-RR (same clone as 83266-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83266-5-RR (BUB3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83266-5-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 83266-5-RR (same clone as 83266-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BUB3 antibody (83266-5-RR, Clone: 240087D9 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83266-5-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 83266-5-RR (same clone as 83266-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BUB3 antibody (83266-5-RR, Clone: 240087D9 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83266-5-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 83266-5-RR (same clone as 83266-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BUB3 antibody (83266-5-RR, Clone: 240087D9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83266-5-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83266-5-RR (same clone as 83266-5-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug BUB3 Recombinant antibody (83266-5-RR, Clone:240087D9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83266-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83266-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83266-5-RR against Human BUB3 were performed. The affinity constant is 0.228 nM.

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Geprüfte Anwendungen

Produktinformation

83266-5-PBS bindet in WB, IF/ICC, FC (Intra), ELISA BUB3 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: BUB3 fusion protein Ag25608
• Vollständiger Name: budding uninhibited by benzimidazoles 3 homolog (yeast)
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 37-40 kDa
• GenBank-Zugangsnummer: BC005138
• Gene symbol: BUB3
• Gene ID (NCBI): 9184
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.