BUD31 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

BUD31 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, RIP, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 11798-1-AP

Synonyme

BUD31, BUD31 homolog (S. cerevisiae), EDG 2, EDG2, fSAP17, G10, Protein BUD31 homolog, Protein EDG 2, Protein G10 homolog, YCR063W

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 11798-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11798-1-AP (BUD31 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11798-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11798-1-AP (BUD31 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of hESC using 11798-1-AP

  hESC cells were subjected to SDS PAGE followed by western blot with 11798-1-AP (BUD31 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11798-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11798-1-AP (BUD31 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 11798-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11798-1-AP (BUD31 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 11798-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 11798-1-AP (BUD31 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 11798-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 11798-1-AP (BUD31 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11798-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11798-1-AP (BUD31 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 11798-1-AP

  IP Result of anti-BUD31 (IP:11798-1-AP, 4ug; Detection:11798-1-AP 1:1500) with mouse brain tissue lysate 3440ug.

• 

  IHC staining of human colon cancer using 11798-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11798-1-AP (BUD31 antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, hESC-Zellen, humanes Hirngewebe, Jurkat-Zellen, Mausherzgewebe, Mauslebergewebe, Mauslungengewebe, Raji-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below
• RIP: See 2 publications below

Produktinformation

11798-1-AP bindet in WB, RIP, IP, IHC, IF, CoIP, ELISA BUD31 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BUD31 fusion protein Ag2373
• Vollständiger Name: BUD31 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 144 aa, 17 kDa
• Beobachtetes Molekulargewicht: 17-20 kDa
• GenBank-Zugangsnummer: BC022821
• Gene symbol: BUD31
• Gene ID (NCBI): 8896
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Nature; The spliceosome is a therapeutic vulnerability in MYC-driven cancer.; Authors - Tiffany Y-T Hsu ; KD Validated
human	WB	iScience; Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.; Authors - Mio Iwasaki
mouse	WB,RIP	Cell Death Differ; Bud31-mediated alternative splicing is required for spermatogonial stem cell self-renewal and differentiation; Authors - Junchao Qin ; KD Validated
human	IHC,IF,CoIP,RIP	Nat Commun; Splicing factor BUD31 promotes ovarian cancer progression through sustaining the expression of anti-apoptotic BCL2L12; Authors - Zixiang Wang ; KD Validated