BXDC5 Polyklonaler Antikörper

BXDC5 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 33133-1-AP

Synonyme

Brix domain-containing protein 5, Ribosome biogenesis protein RPF1, Ribosome production factor 1, Ribosome Production Factor 1 Homolog, RPF1

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  WB analysis using 33133-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33133-1-AP (BXDC5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 33133-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using BXDC5 antibody (33133-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, U-251-Zellen
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

33133-1-AP bindet in WB, IF/ICC, ELISA BXDC5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BXDC5 fusion protein Ag37505
• Vollständiger Name: brix domain containing 5
• Berechnetes Molekulargewicht: 349 aa, 40 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC016051
• Gene symbol: BXDC5
• Gene ID (NCBI): 80135
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BXDC5 (Brix domain-containing protein 5), also known as ribosome biogenesis protein RPF1, is a protein containing a Brix domain and is localized in the nucleolus. The Brix domain is a region that shares homology with the yeast protein Pitx1 (ribosome biogenesis protein BRX1). BXDC5 is a key factor in ribosome biogenesis, participating in the maturation of the 60S ribosomal subunit. This protein exerts its function by binding to specific rRNA structures and acts as a downstream effector molecule of the Ran signaling pathway, involved in ribosome biogenesis. In colorectal cancer (CRC), BXDC5 influences the occurrence and development of tumors by regulating ribosome biogenesis.