Band 3/AE 1 Rekombinanter Antikörper

Band 3/AE 1 Rekombinant Antikörper für IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3K21

Kat-Nr. : 81066-1-PBS

Synonyme

Band 3 / AE1, band 3, band 3/ AE1, SLC4A1, Anion exchanger 1

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  IHC staining of human placenta using 81066-1-RR (same clone as 81066-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 81066-1-RR (SLC4A1 antibody) at dilution of 1:1700 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81066-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 81066-1-RR (same clone as 81066-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 81066-1-RR (SLC4A1 antibody) at dilution of 1:1700 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81066-1-PBS in a different storage buffer formulation.

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  IF Staining of human placenta using 81066-1-RR (same clone as 81066-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using Band 3/ AE1 antibody (81066-1-RR, Clone: 3K21 ) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81066-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human placenta using 81066-1-RR (same clone as 81066-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using Band 3/ AE1 antibody (81066-1-RR, Clone: 3K21 ) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81066-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human placenta using 81066-1-RR (same clone as 81066-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Band 3 / AE1 antibody (81066-1-RR, Clone: 3K21 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81066-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human placenta using 81066-1-RR (same clone as 81066-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Band 3 / AE1 antibody (81066-1-RR, Clone: 3K21 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81066-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81066-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81066-1-RR against Human Band 3/AE 1 were performed. The affinity constant is 23.6 pM.

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Geprüfte Anwendungen

Produktinformation

81066-1-PBS bindet in IHC, IF-P, Indirect ELISA Band 3/AE 1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Band 3/AE 1 fusion protein Ag27878
• Vollständiger Name: solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group)
• Berechnetes Molekulargewicht: 911 aa, 102 kDa
• GenBank-Zugangsnummer: BC101570
• Gene symbol: band 3
• Gene ID (NCBI): 6521
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Anion exchanger 1 (AE1), also known as band 3, is a 95-100 kDa transmembrane glycoprotein which is abundantly expressed in erythrocyte plasma membrane and mediates the electroneutral exchange of Cl- and HCO3-. It contains a 52-55 kDa C-terminal membrane crossing domain and a 43 kDa N-terminal cytoplasmic domain. Degradation of band 3 protein occurred during erythrocyte aging. The oxidative stress can also activate the proteolysis of band 3 through caspases.