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Bcl2 Monoklonaler Antikörper

Bcl2 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B5B9

Kat-Nr. : 66799-1-PBS

Synonyme

Bcl-2, Bcl 2, B cell leukemia/lymphoma 2, 1B5B9

Filter:
  • Western Blot (WB) analysis of various lysates using Bcl2 Monoclonal antibody (66799-1-Ig)

    WB analysis using 66799-1-Ig (same clone as 66799-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66799-1-Ig (Bcl2 antibody) at dilution of 1:1400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66799-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using Bcl2 Monoclonal antibody (66799-1-Ig)

    WB analysis of RAW 264.7 using 66799-1-Ig (same clone as 66799-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66799-1-Ig (Bcl2 antibody) at dilution of 1:1400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66799-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using Bcl2 Monoclonal antibody (66799-1-Ig)

    WB analysis of mouse brain using 66799-1-Ig (same clone as 66799-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66799-1-Ig (Bcl2 antibody) at dilution of 1:1400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66799-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using Bcl2 Monoclonal antibody (66799-1-Ig)

    IF Staining of NIH/3T3 using 66799-1-Ig (same clone as 66799-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Bcl2 antibody (66799-1-Ig, Clone: 1B5B9 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66799-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

66799-1-PBS bindet in WB, IF/ICC, Indirect ELISA Bcl2 und zeigt Reaktivität mit Maus

Getestete Reaktivität Maus
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen Bcl2 fusion protein Ag24261
Vollständiger Name B-cell leukemia/lymphoma 2
Berechnetes Molekulargewicht 26 kDa
Beobachtetes Molekulargewicht 26 kDa
GenBank-ZugangsnummerNM-009741
Gene symbol Bcl2
Gene ID (NCBI) 12043
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Bcl2 is a member of the B cell lymphoma 2 protein family. Members of this family regulate cell death in multiple cell types and can have either proapoptotic or antiapoptotic activities. The protein encoded by this gene inhibits mitochondrial-mediated apoptosis. This protein is an integral outer mitochondrial membrane protein that functions as part of signaling pathway that controls mitochondrial permeability in response to apoptotic stimuli. This protein may also play a role in neuron cell survival and autophagy. Abnormal expression and chromosomal translocations of this gene are associated with cancer progression in numerous tissues.