Bcl2 Monoklonaler Antikörper

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• KD/KO Validated

Bcl2 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1B3F7

Kat-Nr. : 68103-1-PBS

Synonyme

Bcl-2, 1B3F7, B cell leukemia/lymphoma 2, Bcl 2

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  WB analysis using 68103-1-Ig (same clone as 68103-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68103-1-Ig (Bcl2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68103-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 68103-1-Ig (same clone as 68103-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 68103-1-Ig (Bcl2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68103-1-PBS in a different storage buffer formulation.

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  WB analysis of C2C12 using 68103-1-Ig (same clone as 68103-1-PBS)

  C2C12 cells were subjected to SDS PAGE followed by western blot with 68103-1-Ig (Bcl2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68103-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68103-1-Ig (same clone as 68103-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68103-1-Ig (Bcl2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68103-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse spleen using 68103-1-Ig (same clone as 68103-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 68103-1-Ig (Bcl2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68103-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 68103-1-Ig (same clone as 68103-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68103-1-Ig (Bcl2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68103-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 68103-1-Ig (same clone as 68103-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68103-1-Ig (Bcl2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68103-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat skin using 68103-1-Ig (same clone as 68103-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 68103-1-Ig (Bcl2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68103-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat skin using 68103-1-Ig (same clone as 68103-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 68103-1-Ig (Bcl2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68103-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 68103-1-Ig (same clone as 68103-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Bcl2 antibody (68103-1-Ig, Clone: 1B3F7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68103-1-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 68103-1-Ig (same clone as 68103-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Bcl2 antibody (68103-1-Ig, Clone: 1B3F7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 68103-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68103-1-PBS bindet in WB, IHC, IF/ICC, ELISA Bcl2 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Bcl2 fusion protein Ag24261
• Vollständiger Name: B-cell leukemia/lymphoma 2
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: NM-009741
• Gene symbol: Bcl2
• Gene ID (NCBI): 12043
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Bcl2 is a member of the B cell lymphoma 2 protein family. Members of this family regulate cell death in multiple cell types and can have either proapoptotic or antiapoptotic activities. The protein encoded by this gene inhibits mitochondrial-mediated apoptosis. This protein is an integral outer mitochondrial membrane protein that functions as part of signaling pathway that controls mitochondrial permeability in response to apoptotic stimuli. This protein may also play a role in neuron cell survival and autophagy. Abnormal expression and chromosomal translocations of this gene are associated with cancer progression in numerous tissues.