Beclin 1 Monoklonaler Antikörper

Beclin 1 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

64

CloneNo.

1C10C4

Kat-Nr. : 66665-1-Ig

Synonyme

ATG6, Beclin 1, beclin 1, autophagy related, beclin1, BECN1, GT197, Protein GT197, VPS30

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Galerie der Validierungsdaten

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  WB analysis using 66665-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66665-1-Ig (Beclin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 66665-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66665-1-Ig (Beclin 1 antibody) at dilution of 1:500 (under 10x lens).

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  IHC staining of human stomach using 66665-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66665-1-Ig (Beclin 1 antibody) at dilution of 1:500 (under 40x lens).

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  IF Staining of mouse heart using 66665-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 66665-1-Ig (Beclin 1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of mouse heart using 66665-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 66665-1-Ig (Beclin 1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, LNCaP-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Mausherzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 57 publications below
• IHC: See 7 publications below
• IF: See 10 publications below
• IP: See 1 publications below

Produktinformation

66665-1-Ig bindet in WB, IP, IHC, IF, ELISA Beclin 1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Ziege
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Beclin 1 fusion protein Ag1843
• Vollständiger Name: beclin 1, autophagy related
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 52-60 kDa
• GenBank-Zugangsnummer: BC010276
• Gene symbol: BECN1
• Gene ID (NCBI): 8678
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Beclin 1, also known as ATG6 or VPS30, interacts with various cofactors (e.g. Ambra1, Barkor (Atg14), Rubicon, or UVRAG) to regulate the lipid kinase Vps34 and promote the formation of the BECLIN1-Vps34-Vps15 complex, hence inducing autophagy. Its function (via the BH3 domain) is inhibited by Bcl-2 or Bcl-XL. Beclin 1 (BECN1) is a crucial molecule in the control of the autophagic activity, and its activity is regulated by multiple mechanisms, including the post-translational modification, protein-protein interaction, and subcellular localization. It plays a role in crosstalk between apoptosis and autophagy. It has been reported that Beclin 1 can be cleaved into fragments of 50, 37 and 35 kDa during apoptosis. It is involved in many disorders, including neurodegeneration and cancer (tumorigenesis). Beclin 1 is a mammalian tumor suppressor, and its gene is monoallelically deleted in 75% of ovarian, 50% of breast, and 40% of prostate cancers. Decreased expression of Beclin 1 has also been observed in human brain and lung tumors. The level of Beclin 1 was decreased in the affected brain regions of patients with Alzheimer's disease early in the disease process. Recent studies have also shown that gain and loss of Beclin 1 function affects the death of heart cells.

Publikationen

Species	Application	Title
mouse	WB	Gastroenterology; Pancreatic acinar cells-derived sphingosine-1-phosphate contributes to fibrosis of chronic pancreatitis via inducing autophagy and activation of pancreatic stellate cells; Authors - Decai Wang
		Nat Commun; IL-6 regulates autophagy and chemotherapy resistance by promoting BECN1 phosphorylation.; Authors - Fuqing Hu ; KO Validated
human	IF	Nat Commun; A mosquito salivary protein promotes flavivirus transmission by activation of autophagy.; Authors - Peng Sun
mouse	WB,IP	Cell Death Differ; USP11 regulates autophagy-dependent ferroptosis after spinal cord ischemia-reperfusion injury by deubiquitinating Beclin 1; Authors - Yuluo Rong
human	IF	Autophagy; Keratinocyte autophagy enables the activation of keratinocytes and fibroblasts and facilitates wound healing.; Authors - Lei Qiang
rat	WB	Bioact Mater; Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration.; Authors - Liwen Luo