Bestrophin-1 Monoklonaler Antikörper

Bestrophin-1 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4D5F1

Kat-Nr. : 60326-1-PBS

Synonyme

ARB, BEST, BEST1, bestrophin 1, Bestrophin-1, BMD, TU15B, VMD2

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  WB analysis of Y79 using 60326-1-Ig (same clone as 60326-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 60326-1-Ig (Bestrophin-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60326-1-PBS in a different storage buffer formulation.

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  WB analysis of Transfected HEK-293 using 60326-1-Ig (same clone as 60326-1-PBS)

  WB result of Bestrophin-1 antibody (60326-1-Ig; 1:1000; room temperature for 1.5 hours) with negative control and Bestrophin-1 overexpressed Transfected HEK-293 cells. This data was developed using the same antibody clone with 60326-1-PBS in a different storage buffer formulation.

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  IF Staining of Y79 using 60326-1-Ig (same clone as 60326-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Y79 cells using Bestrophin-1 antibody (60326-1-Ig, Clone: 4D5F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60326-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60326-1-PBS bindet in WB, IF/ICC, Indirect ELISA Bestrophin-1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Bestrophin-1 fusion protein Ag15129
• Vollständiger Name: bestrophin 1
• Berechnetes Molekulargewicht: 585 aa, 68 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC015220
• Gene symbol: Bestrophin/BEST1
• Gene ID (NCBI): 7439
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Bestrophin-1 (BEST1) is a 68 kDa transmembrane protein which belongs to the bestrophin family of anion channels. It is predominantly expressed in the basolateral membrane of the retinal pigment epithelium. Studies show that bestrophin 1 functions as a Ca(2+)-dependent Cl(-) channel and modulator of voltage-dependent L-type Ca(2+) channels. It may also contribute to the basolateral cell conductance in airway epithelial cells. This protein is encoded by the VMD2 gene. Mutations in VMD2 can lead to different types of retinal or macular degenerations, including Best vitelliform macular dystrophy (BMD), autosomal recessive bestrophinopathy (ARB), autosomal dominant vitreoretinochoroidopathy (ADVIRC) and adult-onset vitelliform macular dystrophy (AVMD).