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Beta-2-Microglobulin Monoklonaler Antikörper

Beta-2-Microglobulin Monoklonal Antikörper für IF/ICC, FC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IF/ICC, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1H3F4

Kat-Nr. : 68395-1-PBS

Synonyme

B2M, 1H3F4, beta 2 microglobulin, beta 2-Microglobulin, Beta-2-microglobulin form pI 5.3

Filter:
  • Immunofluorescence (IF) / fluorescent staining of NCCIT cells using Beta-2-Microglobulin Monoclonal antibody (68395-1-Ig)

    IF Staining of NCCIT using 68395-1-Ig (same clone as 68395-1-PBS)

    Immunofluorescent analysis of NCCIT cells (non-fixation) using Beta-2-Microglobulin antibody (68395-1-Ig, Clone: 1H3F4 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68395-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Beta-2-Microglobulin Monoclonal antibody (68395-1-Ig)

    IF Staining of A431 using 68395-1-Ig (same clone as 68395-1-PBS)

    Immunofluorescent analysis of A431 cells using Beta-2-Microglobulin antibody (68395-1-Ig, Clone: 1H3F4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68395-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using Beta-2-Microglobulin Monoclonal antibody (68395-1-Ig)

    FC experiment of Jurkat using 68395-1-Ig (same clone as 68395-1-PBS)

    1x2*10^6 Jurkat cells were surface stained with 1 μg Beta-2-Microglobulin Monoclonal antibody (68395-1-Ig, Clone:1H3F4) and CoraLite488-conjugated Goat Anti-Mouse IgG(H+L) (Cat.NO. SA00013-1)(N/A), or 1 μg KLH (66360-1-Ig, Clone: T1F8D3F10) (N/A). Cells were not fixed. This data was developed using the same antibody clone with 68395-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

68395-1-PBS bindet in IF/ICC, FC, ELISA Beta-2-Microglobulin und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen Beta-2-Microglobulin fusion protein Eg31815
Vollständiger Name beta-2-microglobulin
Berechnetes Molekulargewicht 119 aa, 14 kDa
Beobachtetes Molekulargewicht 12-14 kDa
GenBank-ZugangsnummerBC032589
Gene symbol B2M
Gene ID (NCBI) 567
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases. This antibody can be used for staining living cells.