Beta-2-Microglobulin Rekombinanter Antikörper

Beta-2-Microglobulin Rekombinant Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

240660A12

Kat-Nr. : 83683-6-RR

Synonyme

B2M, 240660A12, beta 2 microglobulin, beta 2-Microglobulin, Beta-2-microglobulin form pI 5.3

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  WB analysis using 83683-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83683-6-RR (Beta-2-Microglobulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 83683-6-RR

  IP result of anti-Beta-2-Microglobulin (IP:83683-6-RR, 4ug; Detection:83683-6-RR 1:3000) with A431 cells lysate 2180 ug.

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  IHC staining of human tonsillitis using 83683-6-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83683-6-RR (Beta-2-Microglobulin antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 83683-6-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83683-6-RR (Beta-2-Microglobulin antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83683-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83683-6-RR against Human Beta-2-Microglobulin were performed. The affinity constant is 1.18 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HepG2-Zellen, Jurkat-Zellen, Raji-Zellen, U-937-Zellen
• Erfolgreiche IP: A431-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83683-6-RR bindet in WB, IHC, IP, ELISA Beta-2-Microglobulin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Beta-2-Microglobulin fusion protein Ag4433
• Vollständiger Name: beta-2-microglobulin
• Berechnetes Molekulargewicht: 119 aa, 14 kDa
• Beobachtetes Molekulargewicht: 12 kDa
• GenBank-Zugangsnummer: BC032589
• Gene symbol: B2M
• Gene ID (NCBI): 567
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions due to shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.