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Beta-2-Microglobulin Polyklonaler Antikörper

Beta-2-Microglobulin Polyklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®555 Fluorescent Dye

Kat-Nr. : CL555-13511

Synonyme

B2M, beta 2 microglobulin, Beta-2-Microglobulin, HDCMA22P

Galerie der Validierungsdaten

Filter:
  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite®555-conjugated Beta-2-Microglobulin Polyc (CL555-13511)

    IF Staining of A431 using CL555-13511

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite®555 Beta-2-Microglobulin antibody (CL555-13511) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®555-conjugated Beta-2-Microglobulin Polyc (CL555-13511)

    FC experiment of HeLa using CL555-13511

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human Beta-2-Microglobulin (CL555-13511) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFA431-Zellen
Erfolgreiche Detektion in FCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL555-13511 bindet in IF, FC (Intra) Beta-2-Microglobulin und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Beta-2-Microglobulin fusion protein Ag4433
Vollständiger Name beta-2-microglobulin
Berechnetes Molekulargewicht 119 aa, 14 kDa
Beobachtetes Molekulargewicht 12-14 kDa
GenBank-ZugangsnummerBC032589
Gene symbol B2M
Gene ID (NCBI) 567
Konjugation CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570 nm
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.

Protokolle

Produktspezifische Protokolle
IF protocol for CL555 Beta-2-Microglobulin antibody CL555-13511Protokoll herunterladen
Standard-Protokolle
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