Beta Actin Monoklonaler Antikörper

Beta Actin Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Maus / IgM

Getestete Reaktivität

human, Maus, Pflanze, Ratte, Zebrafisch

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

7D2C10

Kat-Nr. : 60008-1-PBS

Synonyme

ACTB, Actin, bate actin, β actin, Actin cytoplasmic 1

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  WB analysis of multi-cells/tissue using 60008-1-Ig (same clone as 60008-1-PBS)

  Western blot analysis of Beta-actin in various tissues and cell lines using Proteintech antibody 60008-1-Ig at a dilution of 1:5000. Extra bands were detected in some species with unknown reason. This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa cells using 60008-1-Ig (same clone as 60008-1-PBS)

  Western blot of Hela cell with anti-Actin-Beta (60008-1-Ig) at various dilutions. This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  WB analysis of arabidopsis whole plant using 60008-1-Ig (same clone as 60008-1-PBS)

  arabidopsis whole plant tissue were subjected to SDS PAGE followed by western blot with 60008-1-Ig (beta actin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 60008-1-Ig (same clone as 60008-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 60008-1-Ig (ACTB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60008-1-Ig (same clone as 60008-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60008-1-Ig (ACTB antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 60008-1-Ig (same clone as 60008-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60008-1-Ig (ACTB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 60008-1-Ig (same clone as 60008-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 60008-1-Ig (ACTB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  WB analysis of rice whole plant using 60008-1-Ig (same clone as 60008-1-PBS)

  rice whole plant tissue were subjected to SDS PAGE followed by western blot with 60008-1-Ig (beta Actin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 60008-1-Ig (same clone as 60008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 60008-1-Ig (Beta Actin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 60008-1-Ig (same clone as 60008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60008-1-Ig (beta Actin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 60008-1-Ig (same clone as 60008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60008-1-Ig (beta Actin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 60008-1-Ig (same clone as 60008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 60008-1-Ig(ACTB antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 60008-1-Ig (same clone as 60008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 60008-1-Ig(ACTB antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 60008-1-Ig (same clone as 60008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 60008-1-Ig(ACTB antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 60008-1-Ig (same clone as 60008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 60008-1-Ig(ACTB antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 60008-1-Ig (same clone as 60008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 60008-1-Ig(ACTB antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 60008-1-Ig (same clone as 60008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 60008-1-Ig(ACTB antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 60008-1-Ig (same clone as 60008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 60008-1-Ig(ACTB antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 60008-1-Ig (same clone as 60008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 60008-1-Ig(ACTB antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 60008-1-Ig (same clone as 60008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 60008-1-Ig(ACTB antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 60008-1-Ig (same clone as 60008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 60008-1-Ig(ACTB antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary using 60008-1-Ig (same clone as 60008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary using 60008-1-Ig(ACTB antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary using 60008-1-Ig (same clone as 60008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary using 60008-1-Ig(ACTB antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 60008-1-Ig (same clone as 60008-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using Beta Actin antibody (60008-1-Ig, Clone: 7D2C10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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  IF Staining of MDCK using 60008-1-Ig (same clone as 60008-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using Beta Actin antibody (60008-1-Ig, Clone: 7D2C10 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 60008-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60008-1-PBS bindet in WB, IHC, IF/ICC, IF-P, ELISA Beta Actin und zeigt Reaktivität mit human, Maus, Pflanze, Ratten, Zebrafisch

• Getestete Reaktivität: human, Maus, Pflanze, Ratte, Zebrafisch
• Wirt / Isotyp: Maus / IgM
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Beta Actin fusion protein Ag0297
• Vollständiger Name: actin, beta
• Berechnetes Molekulargewicht: 375 aa, 42 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC002409
• Gene symbol: Beta Actin
• Gene ID (NCBI): 60
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Caprylsäure/Ammoniumsulfat-Präzipitation
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.