Beta Actin Rekombinanter Antikörper
Beta Actin Rekombinant Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
Hausschwein, human, Maus, Ratte
Anwendung
WB, IHC, IF/ICC, IP, Indirect ELISA
Konjugation
Unkonjugiert
CloneNo.
4H1
Kat-Nr. : 81115-1-PBS
Synonyme
Geprüfte Anwendungen
Produktinformation
81115-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA Beta Actin und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten
Getestete Reaktivität | Hausschwein, human, Maus, Ratte |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Rekombinant |
Typ | Antikörper |
Immunogen | Beta Actin fusion protein Ag14521 |
Vollständiger Name | actin, beta |
Berechnetes Molekulargewicht | 375 aa, 42 kDa |
Beobachtetes Molekulargewicht | 42 kDa |
GenBank-Zugangsnummer | BC002409 |
Gene symbol | Beta Actin |
Gene ID (NCBI) | 60 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Protein-A-Reinigung |
Lagerungspuffer | PBS only |
Lagerungsbedingungen | Store at -80°C. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Beta Actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40, 31, 15 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins. (9173887, 11217076, 10229193 )