Beta Actin Rekombinanter Antikörper

Beta Actin Rekombinant Antikörper für IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Hund, Maus, Ratte

Anwendung

IF

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

4H1

Kat-Nr. : CL647-81115

Synonyme

ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, PS1TP5BP1, β actin



Geprüfte Anwendungen

Erfolgreiche Detektion in IFMDCK-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-81115 bindet in IF Beta Actin und zeigt Reaktivität mit Hausschwein, human, Hund, Maus, Ratten

Getestete Reaktivität Hausschwein, human, Hund, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Beta Actin fusion protein Ag14521
Vollständiger Name actin, beta
Berechnetes Molekulargewicht 375 aa, 42 kDa
Beobachtetes Molekulargewicht 42 kDa
GenBank-ZugangsnummerBC002409
Gene symbol ACTB
Gene ID (NCBI) 60
Konjugation CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Beta Actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40, 31, 15 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins. (9173887, 11217076, 10229193 )

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 647 Beta Actin antibody CL647-81115Protokoll herunterladen
Standard-Protokolle
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