Beta Arrestin 1 Polyklonaler Antikörper

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• KD/KO Validated

Beta Arrestin 1 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15361-1-PBS

Synonyme

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  WB analysis using 15361-1-AP (same clone as 15361-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15361-1-AP (Beta Arrestin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15361-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 15361-1-AP (same clone as 15361-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 15361-1-AP (ARRB1 antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15361-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U-937 using 15361-1-AP (same clone as 15361-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 15361-1-AP (ARRB1 antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15361-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse lung using 15361-1-AP (same clone as 15361-1-PBS)

  IP result of anti-Beta Arrestin 1 (IP:15361-1-AP, 4ug; Detection:15361-1-AP 1:500) with mouse lung tissue lysate 4000ug. This data was developed using the same antibody clone with 15361-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 15361-1-AP (same clone as 15361-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15361-1-AP (beta Arrestin 1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15361-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 15361-1-AP (same clone as 15361-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15361-1-AP (beta Arrestin 1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15361-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 15361-1-AP (same clone as 15361-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 15361-1-AP (Beta Arrestin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15361-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 15361-1-AP (same clone as 15361-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 15361-1-AP (Beta Arrestin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15361-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 15361-1-AP (same clone as 15361-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15361-1-AP (Beta Arrestin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15361-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 15361-1-AP (same clone as 15361-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15361-1-AP (Beta Arrestin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15361-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 15361-1-AP (same clone as 15361-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15361-1-AP (Beta Arrestin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15361-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 15361-1-AP (same clone as 15361-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15361-1-AP (Beta Arrestin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15361-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15361-1-PBS bindet in WB, IHC, IP, Indirect ELISA Beta Arrestin 1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Beta Arrestin 1 fusion protein Ag7608
• Vollständiger Name: arrestin, beta 1
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 47-55 kDa
• GenBank-Zugangsnummer: BC003636
• Gene symbol: Beta Arrestin 1
• Gene ID (NCBI): 408
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

β-Arrestins (ARRBs), the best known regulators of G protein-coupled receptor signaling, are versatile and multifunctional adapter proteins that regulate diverse cellular functions, including cell growth, apoptosis and immune responses. Overexpression of beta Arrestin 1 has been found in various cancers, indicating it as a potential therapeutic target for cancer treatment. Recently expression of ARRB1 in saliva has been identified as a candidate circadian biomarker. ARRB1 migrated as a doublet of two bands of 45 and 55 kDa (PMID:28947386).