Beta Arrestin 1 Rekombinanter Antikörper

Beta Arrestin 1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240974C3

Kat-Nr. : 83837-1-RR

Synonyme

ARRB1, ARR1, ARR 1, ARB2, ARB1

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  WB analysis using 83837-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83837-1-RR (Beta Arrestin 1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 83837-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83837-1-RR (Arrestin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 83837-1-RR

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 83837-1-RR (Arrestin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83837-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83837-1-RR against Human Beta Arrestin 1 were performed. The affinity constant is 0.759 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Maushirngewebe, Rattenlungengewebe, RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83837-1-RR bindet in WB, ELISA Beta Arrestin 1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Beta Arrestin 1 fusion protein Ag7608
• Vollständiger Name: arrestin, beta 1
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 47-55 kDa
• GenBank-Zugangsnummer: BC003636
• Gene symbol: Beta Arrestin 1
• Gene ID (NCBI): 408
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

β-Arrestins (ARRBs), the best known regulators of G protein-coupled receptor signaling, are versatile and multifunctional adapter proteins that regulate diverse cellular functions, including cell growth, apoptosis and immune responses. Overexpression of beta Arrestin 1 has been found in various cancers, indicating it as a potential therapeutic target for cancer treatment. Recently expression of ARRB1 in saliva has been identified as a candidate circadian biomarker. ARRB1 migrated as a doublet of two bands of 45 and 55 kDa (PMID:28947386).