Beta Arrestin 1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

Beta Arrestin 1 Monoklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H8E4

Kat-Nr. : CL488-67580

Synonyme

ARRB1, ARR1, ARR 1, ARB2, ARB1

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  IF Staining of MCF-7 using CL488-67580

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 Beta Arrestin 1 antibody (CL488-67580, Clone: 1H8E4 ) at dilution of 1:200.

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  FC experiment of HeLa using CL488-67580

  1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Beta Arrestin 1 (CL488-67580, Clone:1H8E4) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-67580 bindet in IF/ICC, FC (Intra) Beta Arrestin 1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Beta Arrestin 1 fusion protein Ag7504
• Vollständiger Name: arrestin, beta 1
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC003636
• Gene symbol: Beta Arrestin 1
• Gene ID (NCBI): 408
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

β-Arrestins (ARRBs), the best known regulators of G protein-coupled receptor signaling, are versatile and multifunctional adapter proteins that regulate diverse cellular functions, including cell growth, apoptosis and immune responses. Overexpression of beta Arrestin 1 has been found in various cancers, indicating it as a potential therapeutic target for cancer treatment. Recently expression of beta Arrestin 1 in saliva has been identified as a candidate circadian biomarker.