Beta Arrestin 2 Monoklonaler Antikörper

Beta Arrestin 2 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1E8F2

Kat-Nr. : 68502-1-PBS

Synonyme

ARRB2, β-Arrestin 2, BARR2, Arrestin beta-2, ARR2

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  WB analysis using 68502-1-Ig (same clone as 68502-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68502-1-Ig (Beta Arrestin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68502-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 68502-1-Ig (same clone as 68502-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 68502-1-Ig (Beta Arrestin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68502-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 68502-1-Ig (same clone as 68502-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68502-1-Ig (Beta Arrestin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68502-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 68502-1-Ig (same clone as 68502-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 68502-1-Ig (Beta Arrestin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68502-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 68502-1-Ig (same clone as 68502-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 68502-1-Ig (Beta Arrestin 2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68502-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 68502-1-Ig (same clone as 68502-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 68502-1-Ig (Beta Arrestin 2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68502-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68502-1-Ig (same clone as 68502-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Beta Arrestin 2 antibody (68502-1-Ig, Clone: 1E8F2 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 68502-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68502-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Beta Arrestin 2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Beta Arrestin 2 fusion protein Ag29807
• Vollständiger Name: arrestin, beta 2
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC007427
• Gene symbol: Beta Arrestin 2
• Gene ID (NCBI): 409
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Beta Arrestin 2 is a member of arrestin/beta-arrestin protein family, which are expressed at high levels in the central nervous system (CNS) and play a critical role in the regulation of G-protein coupled receptors (GPCRs) including MOR. Beta Arrestin 2 is an adaptor protein that is important for the regulation of receptors belonging to both dopaminergic and opioid systems. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors.