Beta Catenin Rekombinanter Antikörper

Beta Catenin Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA, ChIP-qPCR

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte, Zebrafisch

Anwendung

WB, IHC, IF/ICC, Indirect ELISA, ChIP-qPCR

Konjugation

Unkonjugiert

CloneNo.

2J24

Kat-Nr. : 80488-1-PBS

Synonyme

2J24, b cat, B-catenin, beta-Catenin, Catenin beta 1

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  WB analysis using 80488-1-RR (same clone as 80488-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80488-1-RR (Beta Catenin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80488-1-PBS in a different storage buffer formulation.

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  WB analysis of zebrafish using 80488-1-RR (same clone as 80488-1-PBS)

  zebrafish tissue was subjected to SDS PAGE followed by western blot with 80488-1-RR (Beta Catenin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80488-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 80488-1-RR (same clone as 80488-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80488-1-RR (Beta Catenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80488-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 80488-1-RR (same clone as 80488-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80488-1-RR (Beta Catenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80488-1-PBS in a different storage buffer formulation.

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  IHC staining of human rectal cancer using 80488-1-RR (same clone as 80488-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 80488-1-RR (Beta Catenin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80488-1-PBS in a different storage buffer formulation.

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  IHC staining of human rectal cancer using 80488-1-RR (same clone as 80488-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 80488-1-RR (Beta Catenin antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80488-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 80488-1-RR (same clone as 80488-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80488-1-RR (Beta Catenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80488-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 80488-1-RR (same clone as 80488-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80488-1-RR (Beta Catenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80488-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 80488-1-RR (same clone as 80488-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80488-1-RR (Beta Catenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80488-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 80488-1-RR (same clone as 80488-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80488-1-RR (Beta Catenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80488-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 80488-1-RR (same clone as 80488-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Beta Catenin antibody (80488-1-RR, Clone: 2J24 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 80488-1-PBS in a different storage buffer formulation.

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  ChIP experiment of HCT 116 using 80488-1-RR (same clone as 80488-1-PBS)

  Chromatin was prepared from HCT 116 cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of β-Catenin (80488-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 80488-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

80488-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA, ChIP-qPCR Beta Catenin und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten, Zebrafisch

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: catenin (cadherin-associated protein), beta 1, 88kDa
• Berechnetes Molekulargewicht: 781 aa, 86 kDa
• Beobachtetes Molekulargewicht: 92 kDa
• GenBank-Zugangsnummer: BC058926
• Gene symbol: Beta Catenin
• Gene ID (NCBI): 1499
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin is a 92-kDa protein, originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)