Beta Catenin Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Beta Catenin Polyklonal Antikörper für IF/ICC, IF-P

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, IF-P

Konjugation

CoraLite®555 Fluorescent Dye

Kat-Nr. : CL555-51067

Synonyme

b cat, B-catenin, beta-Catenin, Catenin beta 1, Catenin beta-1

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  IF Staining of human liver cancer using CL555-51067

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®555 Beta Catenin antibody (CL555-51067) at dilution of 1:100, CoraLite® Plus 647 CD3 antibody (CL647-60181, Clone: 3F3A1, Magenta), CoraLite® Plus 488 CD163 antibody (CL488-16646, green).

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  IF Staining of human liver cancer using CL555-51067

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®555 Beta Catenin antibody (CL555-51067) at dilution of 1:100, CoraLite® Plus 647 CD3 antibody (CL647-60181, Clone: 3F3A1, Magenta), CoraLite® Plus 488 CD163 antibody (CL488-16646, green).

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  IF Staining of MCF-7 using CL555-51067

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite®555 Beta Catenin antibody (CL555-51067) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: humanes Leberkarzinomgewebe
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL555-51067 bindet in IF/ICC, IF-P Beta Catenin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: catenin (cadherin-associated protein), beta 1, 88kDa
• Berechnetes Molekulargewicht: 781 aa, 86 kDa
• Beobachtetes Molekulargewicht: 92 kDa
• GenBank-Zugangsnummer: BC058926
• Gene symbol: Beta Catenin
• Gene ID (NCBI): 1499
• Konjugation: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin is a 92-kDa protein, originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)