Beta Galactosidase Monoklonaler Antikörper

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• KD/KO Validated

Beta Galactosidase Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4F4F4

Kat-Nr. : 66586-1-PBS

Synonyme

GLB1, 4F4F4, Acid beta-galactosidase, Beta-galactosidase, EBP

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  WB analysis using 66586-1-Ig (same clone as 66586-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66586-1-Ig (Beta Galactosidase antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66586-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 66586-1-Ig (same clone as 66586-1-PBS)

  WB result of Beta Galactosidase antibody (66586-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Beta Galactosidase transfected A549 cells. This data was developed using the same antibody clone with 66586-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66586-1-Ig (same clone as 66586-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66586-1-Ig (Beta Galactosidase antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66586-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66586-1-Ig (same clone as 66586-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66586-1-Ig (Beta Galactosidase antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66586-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66586-1-Ig (same clone as 66586-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Beta Galactosidase antibody (66586-1-Ig, Clone: 4F4F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66586-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66586-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Beta Galactosidase und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Beta Galactosidase fusion protein Ag8069
• Vollständiger Name: galactosidase, beta 1
• Berechnetes Molekulargewicht: 76 kDa
• Beobachtetes Molekulargewicht: 64-66 kDa, 76-85 kDa
• GenBank-Zugangsnummer: BC007493
• Gene symbol: GLB1/Beta-galactosidase
• Gene ID (NCBI): 2720
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GLB1(Beta-galactosidase) is also named as ELNR1 or Lactase. It cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. This protein is identical to the elastin-binding protein (EBP), a major component of the nonintegrin cell surface receptor complex expressed in fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. Defects in GLB1 are the cause of GM1-gangliosidosis type 1 (GM1G1), GM1-gangliosidosis type 2 (GM1G2), GM1-gangliosidosis type 3 (GM1G3) and mucopolysaccharidosis type 4B (MPS4B). GBL1 is synthesized as an 85-kDa precursor that is C-terminally processed into a 64-66 kDa mature form and the released ~20-kDa proteolytic fragment was thought to be degraded (PMID: 10744681). GLB1 has 3 isoforms with MW of 76 kDa, 73 kda and 61 kDa.