Beta TRCP Polyklonaler Antikörper

Beta TRCP Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28393-1-PBS

Synonyme

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  WB analysis using 28393-1-AP (same clone as 28393-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28393-1-AP (Beta TRCP antibody) at dilution of 1:5000 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28393-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 28393-1-AP (same clone as 28393-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28393-1-AP (BETA-TRCP,BTRC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28393-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 28393-1-AP (same clone as 28393-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28393-1-AP (BETA-TRCP,BTRC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28393-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

28393-1-PBS bindet in WB, IHC, Indirect ELISA Beta TRCP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Beta TRCP fusion protein Ag28739
• Vollständiger Name: beta-transducin repeat containing
• Berechnetes Molekulargewicht: 605 aa, 69 kDa
• Beobachtetes Molekulargewicht: 60-65 kDa
• GenBank-Zugangsnummer: BC027994
• Gene symbol: Beta TRCP
• Gene ID (NCBI): 8945
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BTRC, also named as BTRCP, FBW1A, and FBXW1A, is an F-box family protein. It is a Substrate recognition component of an SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. BTRC targets many important proteins with diverse functions, such as p53, HRAS, NF-kappa-B p105 subunit, ATF4, SMAD3, SMAD4, CDC25A, DLG1, FBXO5, and the cell cycle checkpoint protein claspin, for ubiquitin-mediated degradation. It is required for the activation of NFKB-mediated transcription by IL1B, MAP3K14, MAP3K1, IKBKB, and TNF. Required for proteolytic processing of GLI3.