Beta galactosidase Rekombinanter Antikörper

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• KD/KO Validated

Beta galactosidase Rekombinant Antikörper für WB, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240788F1

Kat-Nr. : 83706-6-PBS

Synonyme

GLB1, 240788F1, Acid beta-galactosidase, Beta-galactosidase, EBP

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  WB analysis using 83706-6-RR (same clone as 83706-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83706-6-RR (Beta galactosidase antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83706-6-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 83706-6-RR (same clone as 83706-6-PBS)

  WB result of Beta galactosidase antibody (83706-6-RR; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-Beta galactosidase transfected A549 cells. This data was developed using the same antibody clone with 83706-6-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00647-4

  Sandwich ELISA standard curve of MP00647-4, Human Beta galactosidase Recombinant Matched Antibody Pair - PBS only. 83706-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag7792. 83706-6-PBS was HRP conjugated as the detection antibody. Range: 0.39-12.5 ng/mL.

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  Sample test of MP00647-4

  The mean Beta galactosidase concentration was determined to be 10.08 ng/mL in HeLa cell extract based on a 2.00 mg/mL extract load.

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  IF Staining of HepG2 using 83706-6-RR (same clone as 83706-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Beta galactosidase antibody (83706-6-RR, Clone: 240788F1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83706-6-PBS in a different storage buffer formulation.

• 

  FC experiment of A549 using 83706-6-RR (same clone as 83706-6-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug Beta galactosidase Recombinant antibody (83706-6-RR, Clone:240788F1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83706-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83706-6-PBS

  Biolayer interferometry (BLl) kinetic assays of 83706-6-PBS against Human Beta galactosidase were performed. The affinity constant is below 1 pM.

• 

  Affinity and Kinetic Characterization of 83706-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83706-6-RR against Human Beta galactosidase were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83706-6-PBS bindet in WB, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test Beta galactosidase und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Beta galactosidase fusion protein Ag7792
• Vollständiger Name: galactosidase, beta 1
• Berechnetes Molekulargewicht: 76 kDa
• Beobachtetes Molekulargewicht: 62-67 kDa
• GenBank-Zugangsnummer: BC007493
• Gene symbol: GLB1/Beta-galactosidase
• Gene ID (NCBI): 2720
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GLB1(Beta-galactosidase) is also named as ELNR1 or Lactase. It cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. This protein is identical to the elastin-binding protein (EBP), a major component of the nonintegrin cell surface receptor complex expressed in fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. Defects in GLB1 are the cause of GM1-gangliosidosis type 1 (GM1G1), GM1-gangliosidosis type 2 (GM1G2), GM1-gangliosidosis type 3 (GM1G3) and mucopolysaccharidosis type 4B (MPS4B). GBL1 is synthesized as an 85-kDa precursor that is C-terminally processed into a 64-66 kDa mature form and the released ~20-kDa proteolytic fragment was thought to be degraded (PMID: 10744681). The MW of GLB1 after glycosylation is 100-120 kd. GLB1 is prone to produce homodimers (220-240 kd) and higher multimers (PMID: 3926488). GLB1 has 3 isoforms with MW of 76 kDa, 73 kda, and 61 kDa.