Bif-1 Rekombinanter Antikörper

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• KD/KO Validated

Bif-1 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240623E1

Kat-Nr. : 83715-1-RR

Synonyme

SH3GLB1, 240623E1, Bax interacting factor 1, Bax-interacting factor 1, Bif1

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  WB analysis using 83715-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83715-1-RR (Bif-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 83715-1-RR

  WB result of Bif-1 antibody (83715-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Bif-1 transfected HepG2 cells.

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  IF Staining of HeLa using 83715-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Bif-1 antibody (83715-1-RR, Clone: 240623E1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83715-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83715-1-RR against Human Bif-1 were performed. The affinity constant is 0.71 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293-Zellen, HepG2-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83715-1-RR bindet in WB, IF/ICC, ELISA Bif-1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Bif-1 fusion protein Ag7710
• Vollständiger Name: SH3-domain GRB2-like endophilin B1
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 41 kDa
• GenBank-Zugangsnummer: BC007455
• Gene symbol: Bif-1
• Gene ID (NCBI): 51100
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SH3GLB1 also known as Bif-1, contains an N-terminal domain, a central coiled-coil region, and a C-terminal SH3 domain, but shares no significant homology with members of the Bcl-2 family. SH3GLB1 is involved in activation of caspase-dependent apoptosis by promoting BAX/BAK1 activation (PubMed:16227588).