Biglycan Polyklonaler Antikörper

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• KD/KO Validated

Biglycan Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16409-1-PBS

Synonyme

BGN, DSPG1, PG S1, PGI, PG-S1

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  WB analysis using 16409-1-AP (same clone as 16409-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16409-1-AP (Biglycan antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16409-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 16409-1-AP (same clone as 16409-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16409-1-AP (BGN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16409-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 16409-1-AP (same clone as 16409-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16409-1-AP (BGN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16409-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skeletal muscle using 16409-1-AP (same clone as 16409-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16409-1-AP (BGN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16409-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig cartilage using 16409-1-AP (same clone as 16409-1-PBS)

  pig cartilage tissue were subjected to SDS PAGE followed by western blot with 16409-1-AP (Biglycan Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16409-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 16409-1-AP (same clone as 16409-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16409-1-AP (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16409-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 16409-1-AP (same clone as 16409-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16409-1-AP (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16409-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 16409-1-AP (same clone as 16409-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16409-1-AP (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16409-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 16409-1-AP (same clone as 16409-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16409-1-AP (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16409-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 16409-1-AP (same clone as 16409-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16409-1-AP (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16409-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 16409-1-AP (same clone as 16409-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16409-1-AP (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16409-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 16409-1-AP (same clone as 16409-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16409-1-AP (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16409-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 16409-1-AP (same clone as 16409-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 16409-1-AP (Biglycan antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16409-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 16409-1-AP (same clone as 16409-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 16409-1-AP (BGN antibody) at dilution of 1:25 (under 10x lens). This data was developed using the same antibody clone with 16409-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 16409-1-AP (same clone as 16409-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 16409-1-AP (BGN antibody) at dilution of 1:25 (under 40x lens). This data was developed using the same antibody clone with 16409-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 16409-1-AP (same clone as 16409-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16409-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 16409-1-AP (same clone as 16409-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16409-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 16409-1-AP (same clone as 16409-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16409-1-AP (BGN antibody) at dilution of 1:25 (under 10x lens). This data was developed using the same antibody clone with 16409-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 16409-1-AP (same clone as 16409-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16409-1-AP (BGN antibody) at dilution of 1:25 (under 40x lens). This data was developed using the same antibody clone with 16409-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human renal cell carcinoma using 16409-1-AP (same clone as 16409-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16409-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human renal cell carcinoma using 16409-1-AP (same clone as 16409-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16409-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 16409-1-AP (same clone as 16409-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16409-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 16409-1-AP (same clone as 16409-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16409-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 16409-1-AP (same clone as 16409-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16409-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 16409-1-AP (same clone as 16409-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16409-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 16409-1-AP (same clone as 16409-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Biglycan antibody (16409-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16409-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16409-1-PBS bindet in WB, IHC, IF/ICC, ELISA Biglycan und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Biglycan fusion protein Ag0683
• Vollständiger Name: biglycan
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 40-48 kDa
• GenBank-Zugangsnummer: BC004244
• Gene symbol: Biglycan
• Gene ID (NCBI): 633
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Biglycan is a member of the small leucine-rich proteoglycan (SLRP) family (PMID: 18463092). It consists of a core protein of 42 kDa containing leucine-rich repeats (LRRs), to which two chondroitin/dermatan sulfate side chains are covalently bound (PMID: 2212616; 10383378). The tissue-specific chondroitin- or dermatan-sulfate glycosaminoglycan chains of biglycan are attached to amino acid residues at the N-terminus of the core protein (PMID: 22821552). Biglycan is a ubiquitous component of connective tissue matrix and may act as a signaling molecule (PMID: 22821552). Upregulation of biglycan has been reported in multiple types of solid cancer (PMID: 32194659).