Biglycan Monoklonaler Antikörper

Biglycan Monoklonal Antikörper für Indirect ELISA, IHC, WB

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus

Anwendung

Indirect ELISA, IHC, WB

Konjugation

Unkonjugiert

CloneNo.

1E3F2

Kat-Nr. : 67275-1-PBS

Synonyme

BGN, 1E3F2, PG-S1, PGI, PG S1

---

• 

  WB analysis of pig skin using 67275-1-Ig (same clone as 67275-1-PBS)

  pig skin tissue were subjected to SDS PAGE followed by western blot with 67275-1-Ig (Biglycan antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 67275-1-Ig (same clone as 67275-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 67275-1-Ig (Biglycan antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 67275-1-Ig (same clone as 67275-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67275-1-Ig (Biglycan antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig colon using 67275-1-Ig (same clone as 67275-1-PBS)

  pig colon tissue were subjected to SDS PAGE followed by western blot with 67275-1-Ig (Biglycan antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 67275-1-Ig (same clone as 67275-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 67275-1-Ig (same clone as 67275-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 67275-1-Ig (same clone as 67275-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 67275-1-Ig (same clone as 67275-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 67275-1-Ig (same clone as 67275-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 67275-1-Ig (same clone as 67275-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67275-1-Ig (same clone as 67275-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67275-1-Ig (same clone as 67275-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 67275-1-Ig (same clone as 67275-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 67275-1-Ig (same clone as 67275-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 67275-1-Ig (same clone as 67275-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 67275-1-Ig (same clone as 67275-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 67275-1-Ig (same clone as 67275-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 67275-1-Ig (same clone as 67275-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 67275-1-Ig (same clone as 67275-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug Biglycan Monoclonal antibody (67275-1-Ig, Clone:1E3F2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

67275-1-PBS bindet in Indirect ELISA, IHC, WB Biglycan und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Biglycan fusion protein Ag8012
• Vollständiger Name: biglycan
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC004244
• Gene symbol: Biglycan
• Gene ID (NCBI): 633
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Biglycan is a member of the small leucine-rich proteoglycan (SLRP) family (PMID: 18463092). It consists of a core protein of 42 kDa containing leucine-rich repeats (LRRs), to which two chondroitin/dermatan sulfate side chains are covalently bound (PMID: 2212616; 10383378). The tissue-specific chondroitin- or dermatan-sulfate glycosaminoglycan chains of biglycan are attached to amino acid residues at the N-terminus of the core protein (PMID: 22821552). Biglycan is a ubiquitous component of connective tissue matrix and may act as a signaling molecule (PMID: 22821552). Upregulation of biglycan has been reported in multiple types of solid cancer (PMID: 32194659).