Biotin Rekombinanter Antikörper

Biotin Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

n/a

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

242355B1

Kat-Nr. : 98598-1-RR

Synonyme

Vitamin B7, Vitamin H

---

• 

  FC experiment of human PBMCs using 98598-1-RR

  1x10^6 human PBMCs were surface stained with Biotin Anti-Human CD13 (WM15) (Biotin-65186, Clone: WM15), and then stained with 0.25 ug Anti--Biotin Rabbit RecAb (98598-1-RR, Clone: 242355B1) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98598-1-RR

  1x10^6 human PBMCs were surface stained with Biotin Anti-Human CD13 (WM15) (Biotin-65186, Clone: WM15) and then stained with 0.25 ug Anti-Biotin Rabbit RecAb (98598-1-RR, Clone:242355B1) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98598-1-RR

  1x10^6 mouse splenocytes were surface stained with FcZero-rAb™ Biotin Anti-Mouse CD4 (RM4-4) Rabbit IgG Recombinant Antibody (Biotin-FcA65655, Clone: RM4-4), and then stained with 0.25 ug Anti--Biotin Rabbit RecAb (98598-1-RR, Clone:242355B1) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98598-1-RR

  1x10^6 mouse splenocytes were surface stained with FcZero-rAb™ Biotin Anti-Mouse CD4 (RM4-4) Rabbit IgG Recombinant Antibody (Biotin-FcA65655, Clone: RM4-4) and then stained with 0.25 ug Anti--Biotin Rabbit RecAb (98598-1-RR, Clone: 242355B1) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs, Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98598-1-RR bindet in FC Biotin und zeigt Reaktivität mit n/a

• Getestete Reaktivität: n/a
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Biotin-KLH
• Vollständiger Name: Biotin
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

Biotin, also called vitamin H or vitamin B7, is a water-soluble vitamin required by all organisms (PMID: 12055344). It serves as a necessary cofactor for enzymes involved in carboxylation reactions essential for gluconeogenesis, lipid metabolism, and amino acid catabolism (PMID: 38825069). Biotin is often used to label proteins and nucleic acids. This anti-biotin antibody can be used for detecting biotinylated molecules.