Phospho-CD117/c-Kit (Tyr703) Rekombinanter Antikörper

Phospho-CD117/c-Kit (Tyr703) Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

2G1

Kat-Nr. : 83925-1-PBS

Synonyme

CD117, KIT, EC:2.7.10.1, c-Kit, C Kit

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  WB analysis of Mo7e using 83925-1-RR (same clone as 83925-1-PBS)

  Non-treated Mo7e cells and SCF(HZ-1024) treated Mo7e cells were subjected to SDS PAGE followed by western blot with 83925-1-RR (Phospho-CD117/c-Kit (Tyr703) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 83925-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

83925-1-PBS bindet in WB, ELISA Phospho-CD117/c-Kit (Tyr703) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• Berechnetes Molekulargewicht: 976 aa, 110 kDa
• Beobachtetes Molekulargewicht: 140-160 kDa
• GenBank-Zugangsnummer: BC071593
• Gene symbol: CD117
• Gene ID (NCBI): 3815
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Kit is composed of five N-glycosylated immunoglobulin domains in the N-terminal extracellular portion that bind SCF, a transmembrane domain, and the carboxy-terminal intracellular tyrosine kinase domain. The binding of SCF autophosphorylates Kit on specific tyrosine residues (Tyr), such as Tyr568/570, Tyr703, Tyr721, and Tyr936. Kit then binds to other cytoplasmic enzymes, such as PI3K and Src kinases, and this complex activates downstream molecules. This activates the Akt-Bad pathway and the Ras-Mek-Erk cascade, which regulate gene expression and cytoskeletal structures, resulting in cell proliferation and survival. (PMID: 28403213)