C-Reactive Protein/CRP Rekombinanter Antikörper

C-Reactive Protein/CRP Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240759B8

Kat-Nr. : 83741-3-RR

Synonyme

PTX1, PTX 1, CRP, 240759B8

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  WB analysis of rat liver using 83741-3-RR

  rat liver tissue were subjected to SDS PAGE followed by western blot with 83741-3-RR (C-Reactive Protein/CRP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat liver using 83741-3-RR

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83741-3-RR (C-Reactive Protein/CRP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 83741-3-RR

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83741-3-RR (C-Reactive Protein/CRP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83741-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83741-3-RR against Rat Crp were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenlebergewebe
• Erfolgreiche Detektion in IHC: Rattenlebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83741-3-RR bindet in WB, IHC, ELISA C-Reactive Protein/CRP und zeigt Reaktivität mit Ratten

• Getestete Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: C-reactive protein, pentraxin-related
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: NM_017096.3
• Gene symbol: Crp
• Gene ID (NCBI): 25419
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

C-reactive protein (CRP) is an acute-phase serum protein synthesized by the liver in response to interleukin-6 (IL-6) during inflammation. The name CRP derives from its ability to react with the C polysaccharide of Streptococcus pneumoniae. CRP is an annular, pentameric protein belonging to the pentraxin family. CRP displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis, and complement fixation through its calcium-dependent binding to phosphorylcholine. It is used mainly as a marker of inflammation.