SDH5 Polyklonaler Antikörper

SDH5 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 19906-1-AP

Synonyme

C11orf79, hSDH5, PGL2, SDH assembly factor 2, SDH5, SDHAF2, SDHAF2, PGL2, SDH5

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  WB analysis using 19906-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19906-1-AP (SDH5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 19906-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19906-1-AP (SDH5 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 19906-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19906-1-AP (SDH5 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin cancer using 19906-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 19906-1-AP (SDH5 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin cancer using 19906-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 19906-1-AP (SDH5 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 19906-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SDH5 antibody (19906-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

19906-1-AP bindet in WB, IHC, IF/ICC, ELISA SDH5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SDH5 fusion protein Ag13893
• Vollständiger Name: chromosome 11 open reading frame 79
• Berechnetes Molekulargewicht: 166 aa, 20 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC002331
• Gene symbol: SDH5
• Gene ID (NCBI): 54949
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SDH5, also named asC11orf79, SDHAF2 and SDHAF2, is a mitochondrial protein needed for the flavination of a succinate dehydrogenase complex subunit required for activity of the complex. SDH5 has been shown to be mutated in patients with paragangliomas, also known as familial non-chromaffin paragangliomas type 2. Catalog#19906-1-AP is a rabbit polyclonal antibody raised against the full-length of human SDH5.

Publikationen

Species	Application	Title
human	WB	Free Radic Biol Med; SIRT5-mediated SDHA desuccinylation promotes clear cell renal cell carcinoma tumorigenesis.; Authors - Yuanzhen Ma
human	WB	Apoptosis; GAS6/Axl is associated with AMPK activation and attenuates H2O2-induced oxidative stress; Authors - Zhenxing Liang
mouse	WB	Cell Biochem Funct; Pterostilbene protects against H2 O2 -induced oxidative stress by regulating GAS6/Axl signaling in HL-1 cells; Authors - Wencheng Di
mouse	WB,IHC,IF	Biochim Biophys Acta Mol Basis Dis; Succinate dehydrogenase mediated ROS production contributes to ASIC1a-induced chondrocyte pyroptosis in rheumatoid arthritis; Authors - Wenqiang Liu