ASUN Polyklonaler Antikörper

ASUN Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 19892-1-AP

Synonyme

INTS13, NET48, Integrator complex subunit 13, Germ cell tumor 1, GCT1

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  WB analysis using 19892-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19892-1-AP (ASUN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 19892-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 19892-1-AP (ASUN antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 19892-1-AP

  IP result of anti-ASUN (IP:19892-1-AP, 4ug; Detection:19892-1-AP 1:8000) with MCF-7 cells lysate 1400 ug.

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  IHC staining of human cervical cancer using 19892-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19892-1-AP (ASUN antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human cervical cancer using 19892-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19892-1-AP (ASUN antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 19892-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19892-1-AP (ASUN antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 19892-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19892-1-AP (ASUN antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of MCF-7 using 19892-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ASUN antibody (19892-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, MCF-7-Zellen
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

19892-1-AP bindet in WB, IHC, IF/ICC, IP, ChIP, ELISA ASUN und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ASUN fusion protein Ag13727
• Vollständiger Name: chromosome 12 open reading frame 11
• Berechnetes Molekulargewicht: 706 aa, 80 kDa
• Beobachtetes Molekulargewicht: 70-80 kDa
• GenBank-Zugangsnummer: BC003081
• Gene symbol: ASUN
• Gene ID (NCBI): 55726
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Asunder (ASUN) is a conserved protein that has been reported as a critical regulator of dynein localization during Drosophila spermatogenesis (PMID: 19357193). Mammalian and Drosophila ASUN proteins revealed that they are 43% identical and 64% similar. Human ASUN (also known as GCT1 or C12ORF11) promotes dynein recruitment and centrosomal tethering to the nucleus at mitotic entry (PMID: 23097494).

Publikationen

Species	Application	Title
human	IF	Mol Cell; Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.; Authors - Hagai Marmor-Kollet
human	WB	Nat Commun; INTS13 variants causing a recessive developmental ciliopathy disrupt assembly of the Integrator complex; Authors - Lauren G. Mascibroda
human	ChIP, IP, WB	Nat Commun; Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes.; Authors - Bernd Stadelmayer
human	WB	Int J Clin Exp Pathol; Quantitative proteomic analysis identifies new effectors of FOXM1 involved in breast cancer cell migration.; Authors - Xiaojuan Ye