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ASUN Rekombinanter Antikörper

ASUN Rekombinant Antikörper für WB, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241586E9

Kat-Nr. : 84252-4-PBS

Synonyme

INTS13, Integrator complex subunit 13, Germ cell tumor 1, GCT1, C12orf11

Filter:
  • Western Blot (WB) analysis of various lysates using ASUN Recombinant antibody (84252-4-RR)

    WB analysis using 84252-4-RR (same clone as 84252-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84252-4-RR (C12orf11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84252-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ASUN Recombinant antibody (84252-4-RR)

    IF Staining of HeLa using 84252-4-RR (same clone as 84252-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ASUN antibody (84252-4-RR, Clone: 241586E9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84252-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01142-1

    Cytometric bead array standard curve of MP01142-1

    Cytometric bead array standard curve of MP01142-1, ASUN Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84252-4-PBS. Detection antibody: 84252-3-PBS. Standard: Ag13727. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84252-4-RR

    Affinity and Kinetic Characterization of 84252-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84252-4-RR against Human ASUN were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

84252-4-PBS bindet in WB, IF/ICC, Cytometric bead array, Indirect ELISA ASUN und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen ASUN fusion protein Ag13727
Vollständiger Name chromosome 12 open reading frame 11
Berechnetes Molekulargewicht 706 aa, 80 kDa
Beobachtetes Molekulargewicht70-80 kDa
GenBank-ZugangsnummerBC003081
Gene symbol ASUN
Gene ID (NCBI) 55726
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Asunder (ASUN) is a conserved protein that has been reported as a critical regulator of dynein localization during Drosophila spermatogenesis (PMID: 19357193). Mammalian and Drosophila ASUN proteins revealed that they are 43% identical and 64% similar. Human ASUN (also known as GCT1 or C12ORF11) promotes dynein recruitment and centrosomal tethering to the nucleus at mitotic entry (PMID: 23097494).