C12orf29 Polyklonaler Antikörper

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• KD/KO Validated

C12orf29 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31647-1-PBS

Synonyme

RNA Ligase, RNA Ligase 1, Rnl

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  WB analysis using 31647-1-AP (same clone as 31647-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31647-1-AP (C12orf29 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31647-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 31647-1-AP (same clone as 31647-1-PBS)

  WB result of C12orf29 antibody (31647-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C12orf29 transfected HeLa cells. This data was developed using the same antibody clone with 31647-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 31647-1-AP (same clone as 31647-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 31647-1-AP (C12orf29 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31647-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse small intestine using 31647-1-AP (same clone as 31647-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 31647-1-AP (C12orf29 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31647-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 31647-1-AP (same clone as 31647-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using C12orf29 antibody (31647-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 31647-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

31647-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA C12orf29 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C12orf29 fusion protein Ag35681
• Vollständiger Name: chromosome 12 open reading frame 29
• Berechnetes Molekulargewicht: 37kDa，325aa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: NM_001009894.2
• Gene symbol: C12orf29
• Gene ID (NCBI): 91298
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

C12orf29, also known as RLIG1, is a 37 kDa human protein consisting of 325 amino acids. It catalyzes ATP-dependent RNA ligation via a three-step mechanism involving tandem auto- and RNA AMPylation (PMID: 36792600).