C12orf30 Polyklonaler Antikörper

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• KD/KO Validated

C12orf30 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31748-1-AP

Synonyme

MDM20, NAA25, N-alpha-acetyltransferase 25, NatB auxiliary subunit, NAP1, N-terminal acetyltransferase B complex subunit MDM20

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  WB analysis of HeLa using 31748-1-AP

  WB result of C12orf30 antibody (31748-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C12orf30 transfected HeLa cells.

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  WB analysis using 31748-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31748-1-AP (C12orf30 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse ovary using 31748-1-AP

  IP result of anti-C12orf30 (IP:31748-1-AP, 4ug; Detection:31748-1-AP 1:1500) with mouse ovary tissue lysate 640 ug.

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  IP experiment of HeLa using 31748-1-AP

  IP result of anti-C12orf30 (IP:31748-1-AP, 4ug; Detection:31748-1-AP 1:2000) with HeLa cells lysate 1280 ug.

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  IHC staining of human cervical cancer using 31748-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 31748-1-AP (C12orf30 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 31748-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 31748-1-AP (C12orf30 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen
• Erfolgreiche IP: Maus-Eierstockgewebe, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

31748-1-AP bindet in WB, IHC, IP, ELISA C12orf30 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C12orf30 fusion protein Ag36383
• Vollständiger Name: chromosome 12 open reading frame 30
• Beobachtetes Molekulargewicht: 113 kDa，140 kDa
• GenBank-Zugangsnummer: BC113585
• Gene symbol: C12orf30
• Gene ID (NCBI): 80018
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The C12orf30 gene encodes an auxiliary subunit of the N-terminal acetyltransferase B (NatB) complex, which is responsible for the acetylation of methionine residues at the N-terminus of proteins. The C12orf30 protein plays a role in the distribution and morphology of organelles, and is involved in cell migration and the dynamic changes of the cytoskeleton. In addition, polymorphisms in the C12orf30 gene are associated with susceptibility to various autoimmune diseases, such as rheumatoid arthritis, juvenile idiopathic arthritis, and type 1 diabetes. The C12orf30 protein also participates in regulating the function of immune cells, influencing their signal transduction. The theoretical size of C12orf30 protein is 113 kDa, and it is possible that post-translational modifications may have shifted the protein band up to about 140 kD.