C12orf30 Polyklonaler Antikörper

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• KD/KO Validated

C12orf30 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31748-1-PBS

Synonyme

MDM20, NAA25, N-alpha-acetyltransferase 25, NatB auxiliary subunit, NAP1, N-terminal acetyltransferase B complex subunit MDM20

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  WB analysis of HeLa using 31748-1-AP (same clone as 31748-1-PBS)

  WB result of C12orf30 antibody (31748-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C12orf30 transfected HeLa cells. This data was developed using the same antibody clone with 31748-1-PBS in a different storage buffer formulation.

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  WB analysis using 31748-1-AP (same clone as 31748-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31748-1-AP (C12orf30 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31748-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse ovary using 31748-1-AP (same clone as 31748-1-PBS)

  IP result of anti-C12orf30 (IP:31748-1-AP, 4ug; Detection:31748-1-AP 1:1500) with mouse ovary tissue lysate 640 ug. This data was developed using the same antibody clone with 31748-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 31748-1-AP (same clone as 31748-1-PBS)

  IP result of anti-C12orf30 (IP:31748-1-AP, 4ug; Detection:31748-1-AP 1:2000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 31748-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 31748-1-AP (same clone as 31748-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 31748-1-AP (C12orf30 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31748-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 31748-1-AP (same clone as 31748-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 31748-1-AP (C12orf30 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31748-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

31748-1-PBS bindet in WB, IHC, IP, Indirect ELISA C12orf30 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C12orf30 fusion protein Ag36383
• Vollständiger Name: chromosome 12 open reading frame 30
• Beobachtetes Molekulargewicht: 113 kDa，140 kDa
• GenBank-Zugangsnummer: BC113585
• Gene symbol: C12orf30
• Gene ID (NCBI): 80018
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The C12orf30 gene encodes an auxiliary subunit of the N-terminal acetyltransferase B (NatB) complex, which is responsible for the acetylation of methionine residues at the N-terminus of proteins. The C12orf30 protein plays a role in the distribution and morphology of organelles, and is involved in cell migration and the dynamic changes of the cytoskeleton. In addition, polymorphisms in the C12orf30 gene are associated with susceptibility to various autoimmune diseases, such as rheumatoid arthritis, juvenile idiopathic arthritis, and type 1 diabetes. The C12orf30 protein also participates in regulating the function of immune cells, influencing their signal transduction. The theoretical size of C12orf30 protein is 113 kDa, and it is possible that post-translational modifications may have shifted the protein band up to about 140 kD.