C13orf3 Polyklonaler Antikörper

C13orf3 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26320-1-AP

Synonyme

RAMA1, SKA3, Spindle And Kinetochore Associated Complex Subunit 3, Spindle and kinetochore-associated protein 3

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  WB analysis using 26320-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26320-1-AP (C13orf3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HL-60-Zellen, Jurkat-Zellen, K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

26320-1-AP bindet in WB, ELISA C13orf3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C13orf3 fusion protein Ag23684
• Vollständiger Name: chromosome 13 open reading frame 3
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC048988
• Gene symbol: C13orf3
• Gene ID (NCBI): 221150
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

C13orf3 (now known as Ska3) is a component of a spindle- and mitophore-associated protein complex that regulates microtubule attachment to the mitophore during mitosis. Ska3 is localized to the outer side of the mitophore during mitosis and may be essential for normal chromosome segregation. In addition, deletion of Ska3 results in a delay in mid-mitotic division and an inability to silence the spindle checkpoint or enter prophase. Accumulation of Ska3 is dependent on Sgo1 proteins, whereas mitotic accumulation of Sgo1 in prophase is not dependent on Ska3. The discovery of Ska3 further refines the function of the Ska complex, which plays an important role in mitotic progression and chromosome segregation. The discovery of Ska3 in the prophase of Sgo1 is a key factor in mitosis and chromosome segregation.