NRDE2 Polyklonaler Antikörper

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• KD/KO Validated

NRDE2 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IP, CoIP, chIP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 24968-1-AP

Synonyme

C14orf102, Nuclear exosome regulator NRDE2, Protein NRDE2 homolog, UPF0614 protein C14orf102

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  WB analysis of MCF-7 using 24968-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 24968-1-AP (C14orf102 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 24968-1-AP

  IP result of anti-NRDE2 (IP:24968-1-AP, 5ug; Detection:24968-1-AP 1:500) with MCF-7 cells lysate 1200ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen
• Erfolgreiche IP: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

24968-1-AP bindet in WB, IHC, IP, CoIP, chIP, ELISA NRDE2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NRDE2 fusion protein Ag21805
• Vollständiger Name: chromosome 14 open reading frame 102
• Berechnetes Molekulargewicht: 1164 aa, 133 kDa
• Beobachtetes Molekulargewicht: 133 kDa
• GenBank-Zugangsnummer: BC118673
• Gene symbol: NRDE2
• Gene ID (NCBI): 55051
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IP,CoIP	Genes Dev; An Mtr4/ZFC3H1 complex facilitates turnover of unstable nuclear RNAs to prevent their cytoplasmic transport and global translational repression.; Authors - Koichi Ogami
human	WB,chIP	RNA Biol; NRDE-2, the human homolog of fission yeast Nrl1, prevents DNA damage accumulation in human cells.; Authors - Patricia Richard
human	WB	J Virol; Kaposi's Sarcoma-Associated Herpesvirus Fine-Tunes the Temporal Expression of Late Genes by Manipulating a Host RNA Quality Control Pathway.; Authors - Julio C Ruiz ; KD Validated
human	WB,IP,IHC	Cell Genom; NRDE2 deficiency impairs homologous recombination repair and sensitizes hepatocellular carcinoma to PARP inhibitors; Authors - Yahui Wang ; KD Validated
human	WB,IP	Genes Dev; NRDE2 negatively regulates exosome functions by inhibiting MTR4 recruitment and exosome interaction.; Authors - Jianshu Wang ; KD Validated