VIPAS39 Rekombinanter Antikörper

VIPAS39 Rekombinant Antikörper für WB, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241616A12

Kat-Nr. : 84320-2-PBS

Synonyme

C14orf133, 241616A12, hSPE 39, hSPE-39, SPE39

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  WB analysis using 84320-2-RR (same clone as 84320-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84320-2-RR (C14orf133 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84320-2-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 84320-2-RR (same clone as 84320-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using VIPAS39 antibody (84320-2-RR, Clone: 241616A12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84320-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01194-2

  Cytometric bead array standard curve of MP01194-2, VIPAS39 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84320-2-PBS. Detection antibody: 84320-3-PBS. Standard: Ag14762. Range: 0.781-100 ng/mL.

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  Cytometric bead array standard curve of MP01194-3

  Cytometric bead array standard curve of MP01194-3, VIPAS39 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84320-2-PBS. Detection antibody: 84320-1-PBS. Standard: Ag14762. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 84320-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84320-2-RR against Human VIPAS39 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

84320-2-PBS bindet in WB, IF/ICC, Cytometric bead array, Indirect ELISA VIPAS39 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: VIPAS39 fusion protein Ag14762
• Vollständiger Name: chromosome 14 open reading frame 133
• Berechnetes Molekulargewicht: 493 aa, 57 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC015054
• Gene symbol: VIPAS39
• Gene ID (NCBI): 63894
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VIPAS39 (also known as C14orf133 or SPE-39) is a binding protein to Vps33B, one of the subunits in the mammalian HOPS complex. VIPAS39 may be involved in endosomal maturation or fusion (PMID: 23918659). Mutations in Vps33B and VIPAS39 cause arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome, which is a rare autosomal recessive multisystem disorder associated with abnormalities in polarized liver and kidney cells (PMID: 20190753).