C14orf138 Polyklonaler Antikörper

C14orf138 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24722-1-PBS

Synonyme

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  WB analysis of PC-3 using 24722-1-AP (same clone as 24722-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 24722-1-AP (C14orf138 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24722-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 24722-1-AP (same clone as 24722-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24722-1-AP (C14orf138 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24722-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 24722-1-AP (same clone as 24722-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 24722-1-AP (C14orf138 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24722-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 24722-1-AP (same clone as 24722-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24722-1-AP (C14orf138 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24722-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 24722-1-AP (same clone as 24722-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24722-1-AP (C14orf138 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24722-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

24722-1-PBS bindet in WB, IHC, Indirect ELISA C14orf138 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C14orf138 fusion protein Ag20154
• Vollständiger Name: chromosome 14 open reading frame 138
• Berechnetes Molekulargewicht: 229 aa, 26 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC105118
• Gene symbol: C14orf138
• Gene ID (NCBI): 79609
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

C14orf138, also named as VCPKMT or METTL21D, is 229 amino acid protein, which belongs to the methyltransferase superfamily. C14orf138 localizes in the cytoplasm and interacts with ALKBH6. C14orf138 as a Protein-lysine N-methyltransferase that specifically trimethylates 'Lys-315' of VCP/p97; this modification may decrease VCP ATPase activity. C14orf138 exists as five isoforms. The calculated molecular weight of C14orf138 is 26 kDa,but we always detected a 46-50 kDa portein by western blot.