C14orf166 Polyklonaler Antikörper

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• KD/KO Validated

C14orf166 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19848-1-PBS

Synonyme

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  WB analysis using 19848-1-AP (same clone as 19848-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19848-1-AP (C14orf166 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19848-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 19848-1-AP (same clone as 19848-1-PBS)

  WB result of C14orf166 antibody (19848-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C14orf166 transfected A549 cells. This data was developed using the same antibody clone with 19848-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse pancreas using 19848-1-AP (same clone as 19848-1-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 19848-1-AP (C14orf166 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19848-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 19848-1-AP (same clone as 19848-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19848-1-AP (C14orf166 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19848-1-PBS in a different storage buffer formulation.

• 

  IP experiment of Jurkat using 19848-1-AP (same clone as 19848-1-PBS)

  IP result of anti-C14orf166 (IP:19848-1-AP, 3ug; Detection:19848-1-AP 1:700) with Jurkat cells lysate 1080ug. This data was developed using the same antibody clone with 19848-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 19848-1-AP (same clone as 19848-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19848-1-AP (C14orf166 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19848-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 19848-1-AP (same clone as 19848-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19848-1-AP (C14orf166 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19848-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 19848-1-AP (same clone as 19848-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 19848-1-AP (C14orf166 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19848-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 19848-1-AP (same clone as 19848-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19848-1-AP (C14orf166 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19848-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Jurkat using 19848-1-AP (same clone as 19848-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using C14orf166 antibody (19848-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 19848-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

19848-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA C14orf166 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C14orf166 fusion protein Ag13900
• Vollständiger Name: chromosome 14 open reading frame 166
• Berechnetes Molekulargewicht: 244 aa, 28 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC001722
• Gene symbol: C14orf166
• Gene ID (NCBI): 51637
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human CLE/C14orf166 protein is a positive modulator of cellular RNA polymerase. CLE is also shown to interact with the influenza virus polymerase complex and colocalizes with viral ribonucleoproteins, and is required for viral replication. Similarly, CLE is identified as a novel host interacting partners of the mature hepatitis C virus core protein. CLE levels were significantly higher in the serum of patients with PaCa compared with the control group was strongly expressed in tumor cells, suggesting that C14orf166 may be a potential biomarker of pancreatic adenocarcinoma.