Nesprin 3 Monoklonaler Antikörper

Nesprin 3 Monoklonal Antikörper für WB, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

1D1G12

Kat-Nr. : 68420-1-Ig

Synonyme

1D1G12, C14orf139, C14orf49, KASH domain-containing protein 3, KASH3

---

• 

  WB analysis using 68420-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68420-1-Ig (C14orf49 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of mouse testis using 68420-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Nesprin 3 antibody (68420-1-Ig, Clone: 1D1G12 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HeLa using 68420-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Nesprin 3 antibody (68420-1-Ig, Clone: 1D1G12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, C2C12-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, LNCaP-Zellen, MOLT-4-Zellen, PC-12-Zellen, Rattenhodengewebe
• Erfolgreiche Detektion in IF-P: Maushodengewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68420-1-Ig bindet in WB, IF/ICC, IF-P, ELISA Nesprin 3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Nesprin 3 fusion protein Ag26012
• Vollständiger Name: chromosome 14 open reading frame 49
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC146604
• Gene symbol: Nesprin 3
• Gene ID (NCBI): 161176
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nesprin 3 also known as C14orf49, SYNE3, belongs to the Nesprin family. Nesprin-3 is a type II transmembrane protein of the outer nuclear membrane and contains a C-terminal KASH domain and a series of spectrin repeats(PMID: 16330710). Nesprin-3, a protein that links intermediate filaments to the nucleus, regulates endothelial cell morphology, perinuclear cytoskeletal organization, and flow-induced cell polarization and migration. Nesprin-3 is robustly expressed in human aortic endothelial cells and localizes to the nuclear envelope(PMID: 21937718).