C17orf81 Polyklonaler Antikörper

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C17orf81 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 10162-1-AP

Synonyme

MSTP071, MST071, HSPC002, ELP5, Elongator complex protein 5

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  WB analysis of HEK-293 using 10162-1-AP

  WB result of C17orf81 antibody (10162-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C17orf81 transfected HEK-293 cells.

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  WB analysis using 10162-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10162-1-AP (C17orf81 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 10162-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10162-1-AP (C17orf81 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10162-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10162-1-AP (C17orf81 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 10162-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10162-1-AP (C17orf81 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 10162-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10162-1-AP (C17orf81 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, HEK-293-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: Mausherzgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

10162-1-AP bindet in WB, IHC, ELISA C17orf81 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C17orf81 fusion protein Ag0194
• Vollständiger Name: chromosome 17 open reading frame 81
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 37 and 45 kDa
• GenBank-Zugangsnummer: BC002762
• Gene symbol: C17orf81
• Gene ID (NCBI): 23587
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ELP5, also named as C17orf81, DERP6 and S-phase 2 protein, acts as subunit of the RNA polymerase II elongator complex. It's involved in TP53-mediated transcriptional regulation. ELP5 has some isoforms with MW 20 kDa, 30 kDa and 34-37 kDa.

Publikationen

Species	Application	Title
		Cancer Res; Genome-Wide CRISPR Screens Identify Multiple Synthetic Lethal Targets That Enhance KRASG12C Inhibitor Efficacy; Authors - Suman Mukhopadhyay

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