C18orf21 Polyklonaler Antikörper

C18orf21 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24977-1-AP

Synonyme

XTP13, PNAS-131, PNAS-124, HsT3108, HBV X-transactivated gene 13 protein

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  WB analysis of COLO 320 using 24977-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 24977-1-AP (C18orf21 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 24977-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24977-1-AP (C18orf21 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas using 24977-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24977-1-AP (C18orf21 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human thyroid cancer using 24977-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 24977-1-AP (C18orf21 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 24977-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 24977-1-AP (C18orf21 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 24977-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 24977-1-AP (C18orf21 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 24977-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 24977-1-AP (C18orf21 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 24977-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24977-1-AP (C18orf21 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 24977-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24977-1-AP (C18orf21 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of COLO 320 using 24977-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed COLO 320 cells using 24977-1-AP (C18orf21 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen
• Erfolgreiche Detektion in IHC: humanes Urothelkarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Pankreasgewebe, humanes Schilddrüsenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: COLO 320-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

24977-1-AP bindet in WB, IHC, IF/ICC, ELISA C18orf21 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C18orf21 fusion protein Ag21782
• Vollständiger Name: chromosome 18 open reading frame 21
• Berechnetes Molekulargewicht: 220 aa, 25 kDa
• Beobachtetes Molekulargewicht: 25-28 kDa
• GenBank-Zugangsnummer: BC119766
• Gene symbol: C18orf21
• Gene ID (NCBI): 83608
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

C18orf21, also named as XTP13, belongs to the UPF0711 family. The MW is 25-28 kDa in WB detection.