MYDGF/C19orf10 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MYDGF/C19orf10 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 11353-1-AP

Synonyme

C19orf10, MYDGF, Myeloid-derived growth factor, SF20

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Galerie der Validierungsdaten

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  WB analysis using 11353-1-AP

  various cells were subjected to SDS PAGE followed by western blot with 11353-1-AP (MYDGF/C19orf10F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 11353-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 11353-1-AP (MYDGF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11353-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11353-1-AP (MYDGF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 11353-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11353-1-AP (MYDGF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 11353-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11353-1-AP (MYDGF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 11353-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11353-1-AP (MYDGF/C19orf10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 11353-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11353-1-AP (MYDGF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11353-1-AP

  Immunofluorescent analysis of HepG2 cells, using MYDGF/C19orf10 antibody 11353-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DU 145-Zellen, HeLa-Zellen, Jurkat-Zellen, Maus-Kolongewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 5 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Produktinformation

11353-1-AP bindet in WB, IHC, IF, ELISA MYDGF/C19orf10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MYDGF/C19orf10 fusion protein Ag1900
• Vollständiger Name: chromosome 19 open reading frame 10
• Berechnetes Molekulargewicht: 173 aa, 19 kDa
• Beobachtetes Molekulargewicht: 15-19 kDa
• GenBank-Zugangsnummer: BC010129
• Gene symbol: C19orf10
• Gene ID (NCBI): 56005
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Myeloid-derived growth factor (MYDGF), also named as chromosome 19 open reading frame 10 (C19orf10), is an FLS-derived protein that is secreted into the synovial fluid. MYDGF as a paracrine-acting protein secreted from monocytes/macrophages that improves tissue repair and heart function after myocardial infarction. MYDGF has been reported to regulates neutrophil motility in interstitial tissue damage(PMID: 34047769).

Publikationen

Species	Application	Title
mouse	WB,IF	Sci Adv; Myeloid-derived growth factor inhibits inflammation and alleviates endothelial injury and atherosclerosis in mice.; Authors - Biying Meng ; KO Validated
human,mouse,rat	WB,IHC,IF	Kidney Int; Myeloid-derived Growth Factor Deficiency Exacerbates Mitotic Catastrophe of Podocytes in Glomerular Disease.; Authors - Ping Zhan ; KO Validated
human	WB,IHC	Front Oncol; The Clinical Relevance and Tumor Promoting Function of C19orf10 in Kidney Renal Clear Cell Carcinoma.; Authors - Yanxin Lu ; KD Validated
human	IHC,WB	J Cancer; C19orf10 promotes malignant behaviors of human bladder carcinoma cells via regulating the PI3K/AKT and Wnt/β-catenin pathways.; Authors - Shi Li ; KD Validated
human,mouse	WB,IHC,IF	Cell Death Dis; Myeloid-derived growth factor alleviates non-alcoholic fatty liver disease alleviates in a manner involving IKKβ/NF-κB signaling; Authors - Yan Ding