C19orf21 Polyklonaler Antikörper

C19orf21 Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (1)

Kat-Nr. : 26338-1-AP

Synonyme

C19orf21, MISP, Mitotic interactor and substrate of PLK1

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 26338-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26338-1-AP (C19orf21 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 26338-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 26338-1-AP (C19orf21 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 26338-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26338-1-AP (C19orf21 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 26338-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 26338-1-AP (C19orf21 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

26338-1-AP bindet in WB, IF, ELISA C19orf21 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C19orf21 fusion protein Ag23712
• Vollständiger Name: chromosome 19 open reading frame 21
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC052236
• Gene symbol: C19orf21
• Gene ID (NCBI): 126353
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

C19orf21, also named as MISP, plays a role in mitotic spindle orientation and mitotic progression. It may be required for directed cell migration and centrosome orientation. C19orf21 regulates the distribution of dynactin at the cell cortex in a PLK1-dependent manner.

Publikationen

Species	Application	Title
human	WB	Front Oncol; Up-Regulated MISP Is Associated With Poor Prognosis and Immune Infiltration in Pancreatic Ductal Adenocarcinoma.; Authors - Xinyang Huang
human	WB	Cell Death Discov; Long noncoding RNA LINC00284 facilitates cell proliferation in papillary thyroid cancer via impairing miR-3127-5p targeted E2F7 suppression.; Authors - Bin Zhou
mouse	WB,IF	J Vet Med Sci; Mitotic spindle positioning protein (MISP) deficiency exacerbates dextran sulfate sodium (DSS)-induced colitis in mice; Authors - Koki Hiura ; KO Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

anwesh (Verified Customer) (07-29-2019)

The protein was found at the right size and no additional background bands were observed around the size range. Perfect!

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 293T, HaCaT, U2OS

C19orf21 Antibody Western Blot, validation (1:1000 dilution) in 293T, HaCaT, U2OS (Cat no:26338-1-AP)