C19orf43 Polyklonaler Antikörper

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• KD/KO Validated

C19orf43 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 19420-1-AP

Synonyme

TRIR, EC:3.1.13.-, Exoribonuclease TRIR, fSAP18, Telomerase RNA component interacting RNase

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  WB analysis of A431 using 19420-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 19420-1-AP (C19orf43 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 19420-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19420-1-AP (C19orf43 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 19420-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 19420-1-AP (C19orf43 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 19420-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19420-1-AP (C19orf43 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 19420-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19420-1-AP (C19orf43 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 19420-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 19420-1-AP (C19orf43 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat colon using 19420-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 19420-1-AP (C19orf43 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Gliomgewebe, humanes Magenkrebsgewebe, Maus-Kolongewebe, Ratten-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IP: See 1 publications below

Produktinformation

19420-1-AP bindet in WB, IHC, IP, ELISA C19orf43 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C19orf43 fusion protein Ag13747
• Vollständiger Name: chromosome 19 open reading frame 43
• Berechnetes Molekulargewicht: 176 aa, 18 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC000216
• Gene symbol: C19orf43
• Gene ID (NCBI): 79002
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

C19orf43, also named as MGC2803 and fSAP18, is an Uncharacterized protein. It is 176aa,18.4kd. We used the antibody raised from the C19orf43 protein, we get a ~28kd in human placenta, 293 and A431 cell.

Publikationen

Species	Application	Title
human	WB,IP	Cell Rep; TERRA R-loops connect and protect sister telomeres in mitosis; Authors - Samantha Sze ; KD Validated