C19orf57 Polyklonaler Antikörper

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• KD/KO Validated

C19orf57 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25050-1-AP

Synonyme

Pre-T/NK cell-associated protein 3B3, BRME1, Break repair meiotic recombinase recruitment factor 1

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  WB analysis using 25050-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25050-1-AP (C19orf57 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 25050-1-AP

  WB result of C19orf57 antibody (25050-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C19orf57 transfected Jurkat cells.

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  WB analysis of PC-3 using 25050-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25050-1-AP (C19orf57 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A2780 using 25050-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 25050-1-AP (C19orf57 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 25050-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 25050-1-AP (C19orf57 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 25050-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25050-1-AP (C19orf57 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 25050-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25050-1-AP (C19orf57 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 25050-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using C19orf57 antibody (25050-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, A2780-Zellen, A431-Zellen, K-562-Zellen, MOLT-4-Zellen, Maushodengewebe, PC-3-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

25050-1-AP bindet in WB, IHC, IF/ICC, ELISA C19orf57 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C19orf57 fusion protein Ag21745
• Vollständiger Name: chromosome 19 open reading frame 57
• Berechnetes Molekulargewicht: 668 aa, 70 kDa
• Beobachtetes Molekulargewicht: 60-100 kDa
• GenBank-Zugangsnummer: BC119719
• Gene symbol: C19orf57
• Gene ID (NCBI): 79173
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.