C19orf59 Rekombinanter Antikörper

C19orf59 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250141G2

Kat-Nr. : 86266-1-RR

Synonyme

Mast cell-expressed membrane protein 1, MCEMP1

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  WB analysis of THP-1 using 86266-1-RR

  THP-1 cells were subjected to SDS PAGE followed by western blot with 86266-1-RR (C19orf59 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of THP-1 using 86266-1-RR

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using C19orf59 antibody (86266-1-RR, Clone: 250141G2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen
• Erfolgreiche Detektion in IF/ICC: THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86266-1-RR bindet in WB, IF/ICC, ELISA C19orf59 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: C19orf59 fusion protein Ag22616
• Vollständiger Name: chromosome 19 open reading frame 59
• Beobachtetes Molekulargewicht: 22/24 kDa
• GenBank-Zugangsnummer: BC104798
• Gene symbol: MCEMP1
• Gene ID (NCBI): 199675
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The MCEMP1 gene contains seven exons and was mapped to human chromosome 19p13.3. The epitope-tagged MCEMP1 has been expressed in mammalian cells and found to be localized to the cellular membrane with its C-terminus extending to the outside of the membrane and N-terminus into the cytoplasmic compartment. An evidently increased expression of mast cell expression membrane protein 1 (MCEMP1) has been observed in stroke patients and its expression independently improved the discrimination of 1-month outcome, suggesting the functionality of peripheral blood expression of MCEMP1 as a biomarker for stroke prognosis . (PMID: 30883005, PMID: 31104315, PMID: 15953541)