C19orf70 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

C19orf70 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Rezensionen (1)

Kat-Nr. : 25514-1-AP

Synonyme

MIC13, MICOS complex subunit MIC13, MICOS13, QIL1

---

• 

  WB analysis using 25514-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25514-1-AP (C19orf70 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 25514-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25514-1-AP (C19orf70 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 25514-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25514-1-AP (C19orf70 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach using 25514-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25514-1-AP (C19orf70 antibody) at dilution of 1:200 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below

Produktinformation

25514-1-AP bindet in WB, IHC, ELISA C19orf70 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C19orf70 fusion protein Ag22003
• Vollständiger Name: chromosome 19 open reading frame 70
• Berechnetes Molekulargewicht: 118 aa, 13 kDa
• Beobachtetes Molekulargewicht: 13 kDa
• GenBank-Zugangsnummer: BC042386
• Gene symbol: C19orf70
• Gene ID (NCBI): 125988
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

C19orf70, also named as QIL1 and protein P117, belongs to the UPF0433 family. This antibody is specific to C19orf70.

Publikationen

Species	Application	Title
human	WB	EMBO J; Individual cristae within the same mitochondrion display different membrane potentials and are functionally independent.; Authors - Dane M Wolf
mouse	WB	iScience; Protease OMA1 modulates mitochondrial bioenergetics and ultrastructure through dynamic association with MICOS complex.; Authors - Martonio Ponte Viana
mouse	WB	Cytotherapy; Stem cells from human exfoliated deciduous teeth affect mitochondria and reverse cognitive decline in a senescence-accelerated mouse prone 8 model.; Authors - Wenhuan Guo
human	WB	mBio; Listeria monocytogenes Exploits Mitochondrial Contact Site and Cristae Organizing System Complex Subunit Mic10 To Promote Mitochondrial Fragmentation and Cellular Infection.; Authors - Filipe Carvalho ; KD Validated
mouse	WB	Cell Rep; Mitochondrial GCN5L1 coordinates with YME1L and MICOS to remodel mitochondrial cristae in white adipocytes and modulate obesity; Authors - Juan Xu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Zee (Verified Customer) (01-28-2020)

This antibody worked very well in adult mouse heart extracts when I performed western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse heart tissue